TMEM165 Knockout cell line (HCT 116)

TMEM165 Knockout cell line (HCT 116) Order

Catalog#

YKO-XN81111

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

TMEM165 Knockout cell line (HCT 116)

Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

TMEM165

Gene ID

55858

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%McCOY's 5A+10% FBS

Target region

Knockout region: Exon 1 (based on transcript TMEM165-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TMEM165 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT 116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TMEM165 gene knockout TMEM165 Knockout cell line (HCT 116) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

TMEM165 Knockout cell line (HCT 116)

Catalog#

YKO-XN81111

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Related Popular Products

Wild-type Cell Lines

Human Colon Carcinoma Cell line(HCT 116)
inquiry More details

Catalog#: YC-C004

Size: 1*10^6

Instruction:

Gene Knockout kit

EZ-editor™Gene Knockout kit
inquiry More details

Catalog#: MY-001

Size: -

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
inquiry More details

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

KO Cell Lines

TMEM165 Knockout cell line (HeLa)
inquiry More details

Organism: Human

Gene Name: TMEM165

Gene ID: 55858

Size: 1*10^6

Catalog#: YKO-H572

YKO-H572(HeLa)
TMEM165 Knockout cell line (HEK293)
inquiry More details

Organism: Human

Gene Name: TMEM165

Gene ID: 55858

Size: 1*10^6

Catalog#: YKO-XN81110

YKO-XN81110(HEK293)
TMEM165 Knockout cell line (A549)
inquiry More details

Organism: Human

Gene Name: TMEM165

Gene ID: 55858

Size: 1*10^6

Catalog#: YKO-XN81109

YKO-XN81109(A549)

Related Popular Products

Wild-type Cell Lines

Human Colon Carcinoma Cell line(HCT 116)

Price (USD): -

Catalog#: YC-C004

Size: 1*10^6

Instruction:

inquiry More details

Gene Knockout kit

EZ-editor™Gene Knockout kit

Price (USD): 998

Catalog#: MY-001

Size: -

Instruction:

inquiry More details

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

Price (USD): -;-

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

inquiry More details

KO Cell Lines

TMEM165 Knockout cell line (HeLa)

Organism: Human

Gene Name: TMEM165

Gene ID: 55858

Size: 1*10^6

Catalog#: YKO-H572

inquiry More details
TMEM165 Knockout cell line (HEK293)

Organism: Human

Gene Name: TMEM165

Gene ID: 55858

Size: 1*10^6

Catalog#: YKO-XN81110

inquiry More details
TMEM165 Knockout cell line (A549)

Organism: Human

Gene Name: TMEM165

Gene ID: 55858

Size: 1*10^6

Catalog#: YKO-XN81109

inquiry More details

TMEM165 Knockout cell line (HCT 116)

Ubigene